【摘要】：為了克隆麥洼牦牛IL-1β基因并分析其蛋白質(zhì)結(jié)構(gòu)及表達(dá),給研究IL-1β基因的生物學(xué)功能提供理論基礎(chǔ),試驗(yàn)利用RT-PCR方法擴(kuò)增麥洼牦牛IL-1β基因序列,分析IL-1β基因在不同組織中的表達(dá)情況,通過生物學(xué)軟件進(jìn)行核酸序列分析及蛋白質(zhì)結(jié)構(gòu)預(yù)測(cè)。結(jié)果表明:麥洼牦牛IL-1β基因大小為899 bp,Gen Bank登錄號(hào)為KU510225。此序列編碼266個(gè)氨基酸,蛋白質(zhì)分子質(zhì)量為30 684.0 u,不含跨膜區(qū)且沒有信號(hào)肽;蛋白二級(jí)結(jié)構(gòu)以α-螺旋為主,三級(jí)結(jié)構(gòu)與小鼠IL-1β蛋白結(jié)構(gòu)類似。IL-1β基因在各組織中表達(dá)量的順序?yàn)槠⑴K肝臟心臟肺臟腎臟。說(shuō)明試驗(yàn)成功克隆了麥洼牦牛IL-1β基因,在脾臟表達(dá)量高,表明IL-1β基因可能參與機(jī)體的免疫應(yīng)答。
[Abstract]:In order to clone and analyze the protein structure and expression of IL-1 尾 gene in Maiwa yak, and to provide a theoretical basis for studying the biological function of IL-1 尾 gene, RT-PCR was used to amplify the IL-1 尾 gene sequence of Mai WA yak. The expression of IL-1 尾 gene in different tissues was analyzed. Nucleic acid sequence analysis and protein structure prediction were carried out by biological software. The results showed that the size of IL-1 尾 gene of Maiwa yak was 899 bp,Gen Bank and the accession number was KU510225.. This sequence encodes 266 amino acids with a molecular weight of 30 684.0 u, no transmembrane region and no signal peptide. The secondary structure of the protein was 偽 -helix, and the tertiary structure was similar to that of mouse IL-1 尾 protein. The order of expression of IL-1 尾 gene in each tissue was spleen, liver, heart, lung and kidney. The results showed that the IL-1 尾 gene of Maiwa Yak was cloned successfully and expressed in the spleen, indicating that the IL-1 尾 gene might be involved in the immune response of the body.
【作者單位】： 西南民族大學(xué)生命科學(xué)與技術(shù)學(xué)院動(dòng)物遺傳育種國(guó)家民委—教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：四川省教育廳重點(diǎn)項(xiàng)目(16ZA0014) 西南民族大學(xué)中央高校青年教師基金項(xiàng)目(2015NZYQN60)
【分類號(hào)】：S823.85

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