【摘要】：[目的]觀察姜黃素對SW620細胞及裸鼠NKD2、CXCR4表達水平的影響。[方法]通過MTS檢測姜黃素對SW620細胞活性的影響;運用Western blot、Real-time q PCR檢測不同濃度姜黃素對SW620細胞及轉染NKD2 si RNA后SW620細胞內NKD2、CXCR4蛋白和m RNA表達的影響。雄性BALB/c裸鼠8只,隨機分成對照組與姜黃素組,每組4只。采用皮下接種SW620細胞。造模后第7d開始給對照組與姜黃素組分別注射對照液和100mg/kg·d濃度的姜黃素懸液,連續用藥14d。給藥后檢測瘤體體積,通過Real-time q PCR檢測瘤體組織內NKD2及CXCR4表達水平。[結果 ]姜黃素能明顯抑制SW620細胞的增殖活性,顯著性上調SW620細胞內NKD2表達及下調CXCR4的表達,且呈劑量依賴性。轉染后發現能上調姜黃素處理后的腫瘤細胞CXCR4的表達水平。體內實驗表明姜黃素抑瘤率為30.69%。通過q PCR發現瘤體組織中NKD2的表達量增加,CXCR4的表達量顯著性降低。[結論]姜黃素均能從體內外明顯抑制結腸癌細胞SW620的生長,其作用機制可能通過上調抑制基因NKD2的表達,抑制Wnt信號通路,進而下調腫瘤細胞CXCR4表達,從而起到抑制腫瘤侵襲轉移的作用。
[Abstract]:[objective] to observe the effect of curcumin on the expression of NKD2,CXCR4 in SW620 cells and nude mice. [methods] the effect of curcumin on the activity of SW620 cells was detected by MTS, and the expression of NKD2,CXCR4 protein and m RNA in SW620 cells and SW620 cells after NKD2 si RNA transfection was detected by Western blot,Real-time Q PCR. Eight male BALB/c nude mice were randomly divided into control group and curcumin group with 4 rats in each group. SW620 cells were inoculated subcutaneously. The control group and curcumin group were injected curcumin suspension with control solution and 100mg/kg d concentration respectively on the 7th day after modeling. The volume of tumor was measured after administration, and the expression of NKD2 and CXCR4 in tumor tissue was detected by Real-time Q PCR. [results] curcumin significantly inhibited the proliferation of SW620 cells, significantly up-regulated the expression of NKD2 and down-regulated the expression of CXCR4 in SW620 cells in a dose-dependent manner. After transfection, it was found that curcumin could up-regulate the expression of CXCR4 in tumor cells treated with curcumin. The inhibition rate of curcumin was 30.69% in vivo. Q PCR showed that the expression of NKD2 increased and the expression of CXCR4 decreased significantly. [conclusion] curcumin can obviously inhibit the growth of SW620 in colon cancer cells in vitro and in vivo. The mechanism of curcumin can inhibit the expression of Wnt signal pathway by up-regulating the expression of NKD2 gene, and then down-regulate the expression of CXCR4 in tumor cells. Thus it can inhibit the invasion and metastasis of tumor.
【作者單位】： 浙江中醫藥大學第一臨床醫學院;浙江中醫藥大學;浙江省腫瘤醫院浙江省腫瘤醫院中西結合重點實驗室;
【基金】：浙江省醫學衛生平臺計劃(2015DTA005)
【分類號】：R285
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本文編號：2305286