本文選題：重組雙歧桿菌-LTB疫苗 + 免疫組化　； 參考：《重慶醫科大學》2010年碩士論文

【摘要】： 雙歧桿菌(Bifidobacterium)是人類腸道內重要的益生菌,對人體具有很好的保健功能。基于雙歧桿菌表達系統的重組疫苗對人體可以產生良好的保護作用,既能使人體產生抵抗病原菌的抗體,又可以調整腸道內菌群的平衡。因此我們選用雙歧桿菌作為重組疫苗的受體菌。 產腸毒素大腸桿菌(ETEC)是引起腹瀉的主要致病菌。它導致數以萬計的人們尤其是發展中國家的嬰幼兒腹瀉,同時它也是旅行者腹瀉的最常見病原菌。因此,一個安全有效的抵抗ETEC腹瀉的疫苗對公眾健康是非常重要的。 目的:為了進一步研究重組雙歧桿菌-LTB疫苗免疫于機體后引發的免疫反應,對免疫大鼠的抗體分泌部位進行組織細胞定位。 方法:通過ELISA方法,間接的證明了機體產生了IgG抗體和IgA抗體;而通過免疫組化方法,對產生sIgA的組織細胞進行進一步的定位。 結果:免疫后的SD大鼠血清中產生了特異性的抗體IgG,腸道內產生了特異性分泌型抗體sIgA。免疫組化的圖片顯示抗體sIgA主要是由小腸組織產生的,分泌的細胞為小腸絨毛固有層細胞和小腸腺細胞。隨著免疫次數的增加,抗體分泌量明顯增加,疫苗激發的免疫反應使機體大量分泌sIgA。 結論:重組雙歧桿菌-LTB疫苗免疫大鼠后抗體sIgA分泌組織為小腸,分泌細胞為小腸絨毛固有層細胞和小腸腺細胞。
[Abstract]:Bifidobacterium (Bifidobacterium) is an important probiotic in human intestinal tract and has good health function for human body. The recombinant vaccine based on Bifidobacterium expression system can produce a good protective effect on human body, which can make the body produce antibodies against pathogenic bacteria and adjust the balance of bacteria in the intestinal tract. So we choose two pairs. Sod is the recipient of the recombinant vaccine.
Enterotoxigenic Escherichia coli (ETEC) is a major pathogen causing diarrhea. It causes tens of thousands of people, especially in developing countries, and is also the most common pathogen of traveller diarrhoea. Therefore, a safe and effective vaccine against ETEC diarrhea is very important to public health.
Objective: in order to further study the immune response induced by the recombinant Bifidobacterium -LTB Vaccine Immunized by the body, the tissue cell location of the antibody secreting parts of the immune rats was carried out.
Methods: the ELISA method was used to indirectly prove that the body produced IgG and IgA antibodies, and the tissue cells that produced sIgA were further localized by immunohistochemical method.
Results: the specific antibody IgG was produced in the serum of SD rats after immunization, and a specific secretory antibody sIgA. was produced in the intestinal tract. The antibody sIgA was produced mainly by small intestinal tissue, and the secreted cells were small intestinal villus propria cells and small intestinal gland cells. The immune response stimulated by the vaccine increases the secretion of sIgA. by the body.
Conclusion: after immunization with recombinant Bifidobacterium -LTB vaccine, the sIgA secreted tissue was small intestine, and secretory cells were small intestinal villus lamina propria cells and small intestine gland cells.
【學位授予單位】：重慶醫科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R392

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