本文選題：骨髓間充質干細胞 + 表皮干細胞　； 參考：《中南大學》2009年碩士論文

【摘要】： 目的:研究HaCaT細胞誘導MSCs向表皮干細胞分化的機制。 方法:1密度梯度離心法分離、培養MSCs,免疫組織化學法鑒定CD34,CD44和CD45。2用插入式細胞培養器將HaCaT和MSCs共培養,設為實驗組;MSCs單獨培養,設為對照組。分別于第3和第7天免疫組織化學法鑒定CK19,Pan-ck和β1整合素。3試驗分為3組,分別為共培養細胞上清液組,HaCaT上清液組和MSCs上清液組。ELISA法檢測上清液中EGF含量。 結果:1分離出的細胞呈梭形,免疫組織化學染色CD34,CD45表達陰性,CD44表達陽性。2共培養后MSCs免疫組織化學染色CK19,Pan-ck和β1整合素陽性,共培養7天后MSCs CK19,Pan-ck和β1整合素的陽性率均高于共培養3天的(P＜0.05)。3共培養組上清液中EGF均值高于HaCaT組(P＜0.01)。 結論:1 HaCaT細胞可以誘導MSCs向表皮干細胞分化。2 EGF是HaCaT細胞誘導MSCs向表皮干細胞分化的因素之一。
[Abstract]:Objective: to study the mechanism of differentiation of MSCs into epidermal stem cells induced by HaCaT cells. Methods MSCs were isolated and cultured by the density gradient centrifugation method of 1: 1. CD34T CD44 and CD45.2 were identified by immunohistochemical method. HaCaT and MSCs were co-cultured in a plug-in cell culture apparatus. The MSCs were cultured separately in the experimental group and in the control group. On the 3rd and 7th day, CK19 Pan-ck and 尾 1 integrin 3 test were divided into three groups, the supernatant group of co-cultured cells, HaCaT supernatant group and MSCs supernatant group. Elisa was used to detect the content of EGF in supernatant. Results the cells isolated from 1 / 1 were fusiform. The positive expression of CD34, CD45, CD44, CD44, and 尾 1 integrin in MSCs were detected by immunohistochemistry, and the positive expression of CD44 and 尾 1 integrin in MSCs were detected by immunohistochemistry. The positive rates of CK19 Pan-ck and 尾 1 integrin in MSCs co-cultured for 7 days were higher than those in HaCaT group (P < 0.05). Conclusion the differentiation of MSCs into epidermal stem cells induced by 1: 1 HaCaT cells. 2 EGF is one of the factors that induce MSCs to differentiate into epidermal stem cells.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R329

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